To assess the effect of serum and peritoneal fluid of the endometriosis patients, we compare the fertilization rate of the mouse gametes and the embryo growth rate of the mouse 2-cell embryos in the media with body fluids of the healthy control
women
and the endometriosis patients.
Three kinds of peritoneal fluid were made using ultrafiltration; containing only fraction of less than 30,000 dalton, 100,000 dalton and whole peritoneal fluids. 5 IU of pregnant mare serum gonadotropin(PMSG) and hC were injected into the
peritoneal
cavities of female mouse with 48 hr interval. Fifteen hours after the hCG injection, the oocytes of the mouse were obtained. The gametes were incubated in T6 media containing the bovine serum albumin(group A), the peritoneal fluid of control
women
(group B), the whole peritoneal fluid of the patients(group C), the peritoneal fluid of the pateints containing vraction of less than 100,000 dalton(group D), and the peritoneal fluid of the patient containing fraction of less than 30,000 and
dalton(group E).
To assess embryo growth , 40 hours after mating, 2-cell monuse embryos were obtained in the fallopian tubes of themouses. The embryos were cultured in the 7 kinds of media for 72 hours. The media include group A, B, C, D, E (mentioned above) and
T6
media containing serum of patient with endometriosis(Group F) and serum of control group(group G).
The fertilization rate in group C was 17.0% and significantly lower than group A, B, D and E (67.4%, 46.9%, 37.5% and 37.6% respectively, P<0.01), while the embryo growth rate in group C was significantly lower than the others (34.0% in group C
vs.
80%
in group A, 63.2% in group B, 66.7% in group D and 71.5% in group E). The embryo growth rates of group F and G were not significantly different.
From this data, we can postulate that the peritoneal fractions less than 100,000 dalton and the serum ofr the endometriosis patient have little effect on fertilization and embryo development.
|